Imaging is gaining a key role in the understanding of pelvic floor disorders. Three-dimensional endoanal, endorectal and endovaginal ultrasonography, and dynamic transperineal US (DTPUS) are nowadays increasingly used in clinical practice for patients suffering from pelvic floor dysfunctions (fecal and urinary incontinence, pelvic organ pro- lapse, obstructed defecation) and for patients with anal and rectal pathologies, either benign (anorectal sepsis) or malignant (anorectal tumors). These noninvasive techniques not only provide a superior depiction of the pelvic anatomy but also yield unique dynamic information. The new 3D technology is on the front line in the diagnostic and therapeutic workup and is superior to 2D-US, due to the elaboration of images, achieving better accuracy in the diagnosis of complex diseases. This course is organised for all physicians who are involved or interested in pelvic floor disorders: general surgeons, gastroenterologists, radiologists, urologists and gynaecologists. Theoretical session presents the main guidelines and indications for the use of the different US techniques in clinical practice, demonstrating the endosonographic appearance of normal anatomy and common pathologic conditions of the pelvic floor. Practical session is organised in order to provide the participants with hands-on experience in the basic use of US and is designed to closely discuss specific details of the techniques. Finally, the course includes a session of hands-on training on PC-3D view workstation to let each participant review, manipulate, analyse and discuss several clinical cases under experts’ advice. A number of highly regarded specialists in this area have been selected to provide their experiences on the different topics of the Course.